1、 Summary

In recent years, the pollution caused by blue-green algae blooms has led to the problem of microcystin toxin flooding in many water systems. Due to the lack of timely monitoring of pollution changes in water source quality and corresponding control measures, toxins directly exist in drinking water bodies or accumulate in fish or shellfish, and accumulate in the human body through the food chain, resulting in human and animal poisoning and even death. The harm of microcystin toxins to humans and the environment has sounded the alarm for us. The detection of microcystin toxins is urgent, and China has taken measures to address it2007year1the moon1Starting from today, a new standard for drinking water will be implemented, among which the detection of microcystin toxins is noteworthy(GB／T20466—2006)Listed as a water quality testing indicator.

2、 Product Introduction

This kit uses microcystin toxin-LR (MC-LR)giveBSAConjugates are used as coated antigens, and antibodies are employedMC-LRMonoclonal antibodies, as primary antibodies, form a one-step indirect competition methodELISAtest kit This kit can be used for microcystin toxins in water-LRQuantitative testing; Other solid samples(Such as algae, sediment, animal tissue, etc)After sample extraction, this product can also be used for quantitative testing.ELISA，Enzyme Linked Immunosorbent assay-The qualitative and quantitative method of adsorbing soluble antigens or antibodies onto solid phase carriers such as polystyrene for immune reactions.

3、 Working principle

Pre coating microcystin on microporous strips-LRgiveBSAThe conjugate serves as a conjugated antigen, and the microcystin toxin in the sample competes with the conjugated antigen pre coated on the microporous strip for microcystin toxin-LRAntibody, after adding enzyme-linked immunosorbent assay secondary antibody and using substrate for color development, the absorbance value of the sample is negatively correlated with the content of microcystin it contains, and the content of microcystin in the corresponding sample can be obtained by comparing it with the standard curve.

4、 Performance parameters

1、 High sensitivity: lower detection limit0.05 μg/L

2、 Quantitative detection interval0.1 ~ 2.0 μg/L

3、 Strong stability: coefficient of variation<10%

4、 Quick and convenient: simple preprocessing, detection time-40min

5、 Low cost:~30first/sample

6、 High throughput: capable of detecting hundreds of samples simultaneously

7、 Shelf life:4℃Below,>6Month

8、 Adapt to environmental temperature:20-40℃

9、 Strong ability to resist matrix interference.

10All indicators are superior to the national standard "Determination of Microcystis Toxins in Water"(GB/T 20466-2006).

5、 Specificity

The reagent kit has good specificity and shows significant cross reactivity with microcystin toxins containing the fourth arginine; There is no cross reactivity with the fourth non arginine microcystin toxin(CR%≤0.01%).

6、 Reagent composition

1、 operation instructions 1share

2、 96Enzyme linked immunosorbent assay (ELISA) plate(vacuum packing) 1piece(8hole/strip×12strip)

3、 Microplate Sealers1fix

4、MC-LRTRC 6bottle(0μg/L，0.1μg/L，0.2μg/L，0.5μg/L，1.0μg/L，2.0μg/L)

5、 microcystin-LRantibody 1bottle

6、 Enzyme labeled secondary antibody solution 1bottle

7、 Chromogenic substrate solutionA 1bottle

8、 Chromogenic substrate solutionB 1bottle

9、 stop solution 1bottle

10Concentrated washing solution(20×) 1bottle

7、 Result analysis

8、 Testing Platform

9、 Operation process

10、 Related service system

1、 Understand customers' needs for microcystin detection and monitoring; Assist clients in determining reasonable monitoring plans. Negotiate with clients on solutions for immune testing platforms; Assist clients in selecting and purchasing the main equipment required for the platform.

2、 Assist clients in systematically integrating and debugging the immune testing platform; Propose suggestions for optimizing and standardizing the detection platform. Provide theoretical training, practical operation method guidance, data analysis, and output of testing reports to the main operators.

3、 Long term tracking services for the operational effectiveness of the monitoring plan and detection platform; Provide guidance to customers' questions at all times.

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